男女18禁啪啪无遮挡激烈网站,鸭子TV国产在线永久播放,最近中文字幕在线MV视频在线,四川妇女BBBB的创业精神

咨詢熱線

15000266580

當(dāng)前位置:首頁(yè) >產(chǎn)品中心>細(xì)胞庫(kù)>人腫瘤細(xì)胞、癌細(xì)胞>Panc 02.03 人胰腺癌細(xì)胞

Panc 02.03 人胰腺癌細(xì)胞

簡(jiǎn)要描述:CRL-2553 Panc 02.03 人胰腺癌細(xì)胞,
原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種;細(xì)胞庫(kù)管理規(guī)范,
提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和培養(yǎng)條件!

  • 產(chǎn)品型號(hào):
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2024-11-12
  • 訪  問(wèn)  量:1846

產(chǎn)品分類(lèi)

Product Category

詳細(xì)介紹

CRL-2553 Panc 02.03 人胰腺癌細(xì)胞

ATCC® Number: CRL-2553™ Price: $417.00

Designations: Panc 02.03

Depositors: EM Jaffee

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens (human)

Morphology: epithelial

Source: Organ: pancreas

Disease: adenocarcinoma

Cellular Products: cytokeratins 7 and 18 [50655]

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.


CRL-2553 Panc 02.03 人胰腺癌細(xì)胞

Tumorigenic: Yes

Oncogene: K-ras +

Antigen Expression: MHC class I +; MHC class II - [50655]

DNA Profile (STR): Amelogenin: X

CSF1PO: 11,12

D13S317: 12

D16S539: 11

D5S818: 12,13

D7S820: 9,10

THO1: 6

TPOX: 9,12

vWA: 17

Age: 70 years

Gender: female

Ethnicity: White

Comments: Panc 02.03 is a pancreatic adenocarcinoma epithelial cell line derived in 1995 from a primary tumor removed from the head-of-the-pancreas of a female with pancreatic adenocarcinoma.

The cell line exhibits a K-ras oncogene mutation at codon 12 where a GGT --> GAT mutation resulted in substitution of aspartic acid for glycine. [50655]

The cells have a reported plating efficiency of 100%. [50655]

Propagation: ATCC complete growth medium: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate and supplemented with 10 Units/ml human insulin, 85%; fetal bovine serum, 15%

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Subculturing: Protocol:

1.Remove and discard culture medium.

2.Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

3.Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

4.Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

5.Add appropriate aliquots of the cell suspension to new culture vessels.

6.Incubate cultures at 37°C.

Subc*tion Ratio: A subc*tion ratio of 1:2 to 1:4 is recommended

Medium Renewal: Every 2 to 3 days

Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Doubling Time: 28.2 hrs [50655]

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001

recommended serum:ATCC 30-2020

References: 50655: Jaffee EM, et al. Development and characterization of a cytokine-secreting pancreatic adenocarcinoma vaccine from primary tumors for use in clinical trials. Cancer J. Sci. Am. 4: 194-203, 1998. PubMed: 9612602



CRL-2553

來(lái)自ATCC細(xì)胞,所有出入庫(kù)細(xì)胞均經(jīng)過(guò)嚴(yán)格的細(xì)胞質(zhì)量檢測(cè),確保細(xì)胞無(wú)污染,符合檢測(cè)合格標(biāo)準(zhǔn)。歡迎來(lái)詢價(jià)詳談

















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說(shuō)明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫(xiě)阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號(hào)7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
男女18禁啪啪无遮挡激烈网站,鸭子TV国产在线永久播放,最近中文字幕在线MV视频在线,四川妇女BBBB的创业精神

                  av在线视频观看| 蜜臀精品久久久999久久久做爰| 亚洲高清一区二区三区| 精品一区二区久久| GOGO人体艺术| 苍井空波多野结衣| 国产91精品对白露脸全集观看| 丰满的少妇xxxxx野外多毛| 午夜av在线| 精品一区二区视频| 亚洲国产精品成人综合久久久| 欧美人妻少妇一区二区三区| 亚洲AV午夜成人片精品网站| 欧美人与动性xxxxx杂性| 免费看60分钟高潮v片视频| 国产区一区二区三| 欧美激情亚洲天堂| 日韩av自拍偷拍| 亚洲精品v欧美精品v日韩精品| 波多野结AV衣东京热无码专区 | 熟妇人妻AV无码一区二区三区| 欧美日本免费高清一区二区| 《人妻》按摩电影| 亚洲中文字幕久久久| 欧美在线人妻| 久久无码人妻精品一区二区三区| 久久秋霞| 精品一区二区三区四区视频| 少妇一xx88av| 一区二区三区手机在线观看| 亚洲国产123| 凹凸精品熟女也国产在线| 亚洲激情熟女| 少妇18p一区二区三区| 亚洲精品乱码久久久久久蜜桃图片 | 免费国偷拍精品视频| 日韩av在线高清| 草草影院在线免费观看| 极品粉嫩国产48尤物在线播放| 久久亚洲精品国产亚洲老地址| 91成人短视频在线观看|